Chromatorgraphy

In: Science

Submitted By burica225
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Amina Buric 02/25/16 Amina Buric 02/25/16

Experiment 4: Chromatography of Food Dyes
Prof.Wang
Experiment 4: Chromatography of Food Dyes
Prof.Wang

Purpose: The purpose of this experiment is to see what colors are used in our food. With this experiment we are able to use salt solvent to identify what colors are used in different dyes.
Procedure:
1. Gather paper, dyes and pencil. 2. Mark a baseline and 1.5cm dividers and label the colors between the lines. 3. Use the toothpick to put on the color. 4. Mix salt and water and stir until dissolved. 5. Put water and salt mixture in a dish and make sure that the water is not touching the baseline. 6. Let the paper sit in the water until it gets wet 2cm from the top. 7. Take the paper out and measure the distance of the color from the baseline. 8. Record the measurements in data tables and calculate Rf using the formula.
Data and observations:
Exercise 1: Paper Chromatography Data Table 1. Photographs of Chromatograms. Photograph of Chromatogram #1 | | Photograph of Chromatogram #2 | |

Data Table 2. Chromatogram Sheet 1 Data. Sample | Solvent front (mm) | Spot 1 | Spot 2 | Spot 3 | | | mm | Rf | mm | Rf | mm | Rf | Blue 1 | 60 | 54 | .9 | | | | | Blue 2 | 60 | 48 | .8 | | | | | Red 3 | 60 | 42 | .7 | | | | | Red 40 | 60 | 45 | .75 | | | | | Yellow 5 | 60 | 41 | .69 | | | | | Yellow 6 | 60 | 40 | .68 | | | | | KG | 60 | 44 | .73 | 54 | .9 | | | KS | 60 | 44 | .73 | 54 | .9 | | | Data Table 3. Chromatogram Sheet 2 Data. Sample | Solvent front (mm) | Spot 1 | Spot 2 | Spot 3 | | | mm | Rf | mm | Rf | mm | Rf | Store Red | 60 | 42 | .7 | | | | | Store Yellow | 60 | 41 | .69 | | | |…...

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