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Chromatorgraphy

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Amina Buric 02/25/16 Amina Buric 02/25/16

Experiment 4: Chromatography of Food Dyes
Prof.Wang
Experiment 4: Chromatography of Food Dyes
Prof.Wang

Purpose: The purpose of this experiment is to see what colors are used in our food. With this experiment we are able to use salt solvent to identify what colors are used in different dyes.
Procedure:
1. Gather paper, dyes and pencil. 2. Mark a baseline and 1.5cm dividers and label the colors between the lines. 3. Use the toothpick to put on the color. 4. Mix salt and water and stir until dissolved. 5. Put water and salt mixture in a dish and make sure that the water is not touching the baseline. 6. Let the paper sit in the water until it gets wet 2cm from the top. 7. Take the paper out and measure the distance of the color from the baseline. 8. Record the measurements in data tables and calculate Rf using the formula.
Data and observations:
Exercise 1: Paper Chromatography Data Table 1. Photographs of Chromatograms. Photograph of Chromatogram #1 | | Photograph of Chromatogram #2 | |

Data Table 2. Chromatogram Sheet 1 Data. Sample | Solvent front (mm) | Spot 1 | Spot 2 | Spot 3 | | | mm | Rf | mm | Rf | mm | Rf | Blue 1 | 60 | 54 | .9 | | | | | Blue 2 | 60 | 48 | .8 | | | | | Red 3 | 60 | 42 | .7 | | | | | Red 40 | 60 | 45 | .75 | | | | | Yellow 5 | 60 | 41 | .69 | | | | | Yellow 6 | 60 | 40 | .68 | | | | | KG | 60 | 44 | .73 | 54 | .9 | | | KS | 60 | 44 | .73 | 54 | .9 | | | Data Table 3. Chromatogram Sheet 2 Data. Sample | Solvent front (mm) | Spot 1 | Spot 2 | Spot 3 | | | mm | Rf | mm | Rf | mm | Rf | Store Red | 60 | 42 | .7 | | | | | Store Yellow | 60 | 41 | .69 | | | | | Store Green | 60 | 41 | .69 | 54 | .9 | | | Store Blue | 60 | 54 | .9 | | | | | Unknown | 60 | 42 | .7 | 54 | .9 | | | M&M: M –R | 60 | 45 | .75 | | | | | M&M: M –Y | 60 | 40 | .68 | | | | | M&M: M –G | 60 | 41 | .69 | 54 | .9 | | | M&M: M –B | 60 | 54 | .9 | | | | |

Data Table 4. FD&C Dyes in Samples. Sample: | FD&C Dyes in Sample | Kool-Aid Grape ® | Red 40, Blue 1 | Kool-Aid Strawberry ® | Red 40, Blue 1 | Store Red | Red 3 | Store Yellow | Yellow 5 | Store Green | Yellow 5 and Blue 1 | Store Blue | Blue 1 | Unknown Sample | Blue 1 Red3 | M&M®Red | Red 40 | M&M Yellow | Yellow 6 | M&M®Green | Red 40 Blue1 | M&M® Blue | Blue 1 |

Post lab questions:
Questions
A.What might happen you used a pen to mark the baseline on the chromatography paper?

The pen might mix with the colors B.What would happen if you did not stop the chromatograph before the solvent reached the top of the paper?

Spots for the mixtures would start to diffuse.

C.What would happen if the solvent level was above that of the dye spots on the paper at the start of the chromatography? The dye spots would not go up the paper.

D.Why is it important to mark the solvent level on the chromatography paper as soon as you remove it from the Petri dish?
Because color continues advancing while the paper is drying

Conclusion: In this lab exercise I have learned how different colors are used to from different dyes on our food. I have learned how to use chorography to see what color samples match in food dyes that we eat in our candy.…...

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